Nole won the Best Male Tennis Player award at the 23rd annual awards show at the Microsoft Theater on July 15 in Los Angeles.
The ESPY, short for Excellence in Sports Performance Yearly, honors sports achievement in the past calendar year.
Nole came out ahead over his hottest tennis rivals Roger Federer and Stan Wawrinka.
He was also nominated for Best International Athlete (won by Lionel Messi).
Djokovic had previosly won the ESPY Best Male Tennis Player Award for achievements in 2011 and 2012.
The award for Best Female Tennis Player went to Serena Williams.
You can download Nole August desktop calendar here.
Photo: Getty Images