The second Grand Slam of the season, the Roland Garros, is taking place in Paris, from May 22 to June 5, 2016.
Last year Nole won his fourth Rome Masters crown with a 6-4, 6-3 victory over Roger Federer in the final.
World no.1 arrived in Madrid with a 28-2 record on the season. The 2011 champion will open campaign against Borna Coric (CRO).
Novak is a top seed and has a first-round BYE. He will open his campaign against world no.55 Jiri Vesely (CZE).
World no.1 and five-time champion is a top seed and has a first round BYE. He will open title defence against Kyle Edmund.
Novak will begin title defence in Indian Wells in the second round against Bjorn Fratangelo (USA, Q).
World no.1 Novak Djokovic returns to the tennis court on Friday, as Serbia hosts Kazakhstan in the Davis Cup first round tie in Belgrade.
Novak is making his tenth appearance at the Dubai Duty Free Tennis Championships, aiming for his fifth crown.
Top seed and five-time defending champion will open campaign in his 12th Australian Open appearance against world no.51, Hyeon Chung.
Novak is no.1 seed and will open his Qatar ExxonMobil Open campaign against German qualifier Dustin Brown (ATP #118).